Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of m RNA m 6 A writer proteins in Arabidopsis thaliana. N6-adenosine methylation (m 6 A) of m RNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. RůžiÄka, Kamil Zhang, Mi Campilho, Ana Bodi, Zsuzsanna Kashif, Muhammad Saleh, Mária Eeckhout, Dominique El-Showk, Sedeer Li, Hongying Zhong, Silin De Jaeger, Geert Mongan, Nigel P Hejátko, Jan Helariutta, Ykä Fray, Rupert G Identification of factors required for m6 A m RNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI.
Thus, Hakai can affect the oncogenic phenotype both by altering E-cadherin-based intercellular adhesions and by increasing PSF’s ability to bind RNAs that promote cancer-related gene expression. Furthermore, the knockdown of PSF suppressed Hakai-induced cell proliferation. Hakai overexpression enhanced the binding of PSF to mRNAs encoding cancer-related proteins, while knockdown of Hakai reduced the RNA-binding ability of PSF. While PSF can function as a DNA-binding protein with a tumor suppressive function, its association with Hakai promotes PSF’s RNA-binding ability and post-transcriptional influence on target mRNAs. Recently, Hakai was also found to interact with PSF (PTB-associated splicing factor). Hakai, an E3 ubiquitin ligase for the E-cadherin complex, plays a crucial role in lowering cell-cell contacts in epithelial cells, a hallmark feature of tumor progression. Hacking RNA: Hakai promotes tumorigenesis by switching on the RNA-binding function of PSFįigueroa, Angélica Fujita, Yasuyuki Gorospe, Myriam
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